Characterization of Pajaroellobacter abortibovis, the etiologic agent of epizootic bovine abortion. Brooks RS(1), Blanchard MT(1), Clothier. J Vet Diagn Invest. May;14(3) Diagnosis of epizootic bovine abortion in Nevada and identification of the vector. Hall MR(1), Hanks D, Kvasnicka W. Kennedy PC, Casaro AP, Kimsey PB, Bon Durant RH, Bushnell RB, Mitchell GM. The development of the fetal lesions of epizootic bovine abortion (EBA) was.

Author: Kazrazahn Zolok
Country: Bahrain
Language: English (Spanish)
Genre: Technology
Published (Last): 13 November 2016
Pages: 260
PDF File Size: 11.55 Mb
ePub File Size: 19.94 Mb
ISBN: 702-3-63422-621-6
Downloads: 31899
Price: Free* [*Free Regsitration Required]
Uploader: Sabar

Diagnosis of epizootic bovine abortion in Nevada and identification of the vector.

A diagnosis of EBA was established on the basis of histopathology and elevated serum immunoglobulin G, as previously defined abodtion10 Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Novel bacterium in phylum Bacteroidetes.

A specific PCR was developed described in Materials and Methods to detect this deltaproteobacterium in bovine tissues. These preliminary results suggest that the agent is preferentially associated with lymphoid tissues. The gross enlargement of lymph nodes was the result of cortical follicular hyperplasia and histiocytic lymphadenitis.

Epizootic bovine abortion EBAfirst identified in the s, is a major contributor of economic loss to western U. From the first experiment, sequences corresponding to a novel betaproteobacterium and Ochrobactrum anthropi were identified. This study demonstrates a strong association between the presence of a novel deltaproteobacterium and the development of EBA.

Congenital spirochetosis in calves: Thirty-six experimentally infected fetuses were recovered at periods of 29 to days after their dams had been infected by allowing the vector tick Ornithodorus coriaceus to feed on them.

Two different fetal bovine thymic pools containing tissue homogenates from two EBA-positive tester or three EBA-negative driver individual cases were prepared by phenol-chloroform extraction This study establishes the presence of EBA as a cause of late-term abortion in Nevada.

The nucleotide sequence data reported in this paper have been submitted to GenBank and have been assigned the following accession numbers: The novel bacterium described anortion this paper represents only the second member of the delta subdivision of the class Proteobacteria to be a mammalian pathogen the first was Lawsonia intracellularis [ 413 ], the causative epizootiv of porcine proliferative enteropathy.


These data imply a close association between this novel agent and spizootic etiology of EBA. EBA is defined as a specific fetal syndrome characterized by late-term abortion or birth of weak or dead calves aabortion. Amplicons identified on 1. The unique fetal pathology of EBA has classically served as the only definitive basis of diagnosis 9 Efforts are under way to define additional gene sequences of this unique pathogen, to visualize e;izootic agent in affected tissues, and to visually link the rRNA sequence to the bacterium using in situ hybridization techniques on diseased fetal necropsy tissue.

Identification of this vector was facilitated by the demonstration that the geographic distribution of the tick largely parallels that of EBA 14 The recent development of a challenge system abortiion which inoculation of EBA-diseased fetal thymus into pregnant 90 to days of gestation heifers could result in consistent and predictable transmission of the EBA agent has facilitated efforts to define the causative organism Acute necrotizing foci developed in several organs, but most commonly in lymph nodes and spleen.

Three of these heifers were simultaneously treated with penicillin for 1 week and tetracycline for 3 weeks, while the remaining three were left untreated to serve as challenge controls Support Center Support Center. Examination of aborted full-term bovine govine obtained from Nevada ranches revealed gross abnormalities consistent with EBA enlarged lymph nodes, petechial hemorrhages of the oral mucosa and conjunctiva, ascites, and splenohepatomegalyand EBA was confirmed by histologic examination of fetal tissues.

This article has been cited by other articles in PMC. The bacterium was also detected in the argasid tick Ornithodoros coriaceuswhich is the recognized vector of EBA. Instruments were subjected to bleach and heat treatment alcohol burn between collections of individual organs. Etiological studies on epizootic bovine abortion.


There was a problem providing the content you requested

GenBank accession numbers are shown. In the fetuses which were either aborted or carried to term after prolonged infection, acute necrotizing lesions were epixootic on the chronic proliferative fetuses.

Vaccination against epizootic bovine chlamydial abortion.

Second abortuon third repetitions of the shPCR experiments were performed. The agent was detected in thymic tissues from affected fetuses and in the argasid tick vector of EBA. Primers designed in a similar fashion generated a partial sequence of the 23S rRNA gene JQ These sequences, combined with a revised 16S rRNA phylogenic analysis, support the placement of this bacteria as a unique genus separate from Sorangium.

Diagnosis of epizootic bovine abortion in Nevada and identification of the vector.

These primer regions showed the most variation compared with other bacterial sequences in multiple-nucleotide alignments. Widespread inflammatory lesions with a vascular orientation are evident in most tissues. Concurrent treatment with antibiotics at the time of EBA challenge abolished infection of the fetus and therefore implicated a prokaryotic organism as the likely causative agent Published by Elsevier B.

Experimentally induced immunity to chlamydial abortion of cattle.

Although the unusual clinical presentation and unique fetal pathology associated with EBA have been recognized since the s, the identity of the etiologic agent is unknown. In summary, this work demonstrates a close relationship between a novel bacterium and EBA.

National Center for Biotechnology InformationU. Identification and sequencing of the groE operon and flanking genes of Lawsonia intracellularis: Taxonomic outline of the prokaryotic genera, p.